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产品性质
| biological source【生物来源】 | mouse blood |
| growth mode【生长模式】 | Suspension |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Lymphoblast |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Mouse C57b1/6 T cell |
| Cell Line Description【细胞系描述】 | CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media. |
| Application【应用】 | CTLL-2 has been used in CTLL-2 cell proliferation assay to determine the presence of T-cell cytokines. |
| Culture Medium【培养基】 | RPMI 1640 + 2mM Glutamine + 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37℃. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 105 cells/ml. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
