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Cyclohexanone Monooxygenase from Acinetobacter sp.
| 产品编号： | 3073334 |
| 规格： | ≥12 U/mL, ammonium sulfate suspension, recombinant, expressed in E. coli |
| CAS NO： | 52037-90-8 |
| 包装规格： | 1 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
52037-90-8
Cyclohexanone Monooxygenase from Acinetobacter sp.
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 1.14.13.22 ( BRENDA | IUBMB ) |
| MDL number | MFCD00146601 |
| NACRES | NA.54 |
| Application【应用】 | Cyclohexanone monooxygenase has been used in a study that cloned and overexpressed the 2-oxo-Δ(3)-4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase (OTEMO) in Escherichia coli. Cyclohexanone monooxygenase has also been used in a study that investigated the effects of structural modification of the cell wall on the biotransformation capability by recombinant Corynebacterium glutamicum cells. |
| Biochem/physiol Actions【生化/生理作用】 | Purified cyclohexanone monooxygenase has a molecular weight of 59,000 Da. It is a versatile oxygenation catalyst. The enzyme uses the bound FAD-4a-OOH oxygenating intermediate to initiate transfer of oxygen to electrophilic substrate sites. The reaction consequently yields the corresponding sulfoxide and selenoxide products. This enzyme is also capable of oxygenating at nitrogen, trivalent phosphorus, and boron sites in boronic acids. Hence, it is one of the most broad-based flavoprotein oxygenases known. |
| Unit Definition【单位定义】 | 1 unit corresponds to the amount of enzyme which catalyzes the cyclohexanone-stimulated oxidation of 1 μmol of NADPH per minute at pH 9.0 and 30 ℃ |
| Physical form【外形】 | Suspension in 80% saturated ammonium sulfate, 20 mM K-Na-phosphate buffer pH 7, 3.5 mM 1,4-Dithioerythritol (DTE) |
产品性质
| Quality Level【质量水平】 | 200 |
| recombinant【重组】 | expressed in E. coli |
| form【形式】 | ammonium sulfate suspension |
| concentration【浓度】 | ≥12 U/mL |
| storage temp.【储存温度】 | −20℃ |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
