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肠激酶 来源于猪肠
Enterokinase from porcine intestine
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| 产品编号 | 包装单位 | 单价(元) | 国内现货 | 国外库存 | 询价单 |
| 3081080 | 200 UNITS | 13350 |
产品别名
9014-74-8
肠激酶 来源于猪肠
Enterokinase from porcine intestine
Enteropeptidase
肠肽酶
基本信息
| Enzyme Commission (EC) Number【酶学委员会(EC)编号】 | 3.4.21.9 ( BRENDA | IUBMB ) |
| EC number【EC 号】 | 232-761-1 |
| MDL number | MFCD00131020 |
| eCl@ss | 32160410 |
| NACRES | NA.54 |
| Application【应用】 | Enterokinase from porcine intestine has been used in a study to investigate complementary DNA cloning and sequencing of rat enteropeptidase. Enterokinase from porcine intestine has also been used to learn more about the insulinotropic region of the gastric inhibitory polypeptide. The enzyme from Sigma has been used to activate zymogens in order to detect trypsin activity. The study to investigated the structural and evolutionary consequences of unpaired cysteines in trypsinogen. The product has been used to measure trypsin while studying the effect of pesticide induced alterations in gene expression in the lobster, Homarus americanus The enzyme from Sigma has been used to develop a novel assay for measuring levels of lipid-free apoA-I in the presence of lipid-bound apoA-I. Enteropeptidase can specifically cleave human lipid-free apoA-I but not its lipid-bound form resulting in an N-terminal fragment of 22 kDa. It has also been used in a study to examine the effect of calcium and phytic acid on the activation of trypsinogen and the stability of trypsin. |
| Biochem/physiol Actions【生化/生理作用】 | Enterokinase is a membrane bound serine protease that specifically and rapidly converts trypsinogen to trypsin, thereby, triggering the conversion of other zymogens to active enzymes. It has a molecular mass of approximately 150 kDa. The enzyme is a heterodimer consisting of 35-47 kDa subunits. The light and the heavy chains are linked by two disulfide bridges. It is a glycoprotein containing 35% carbohydrate. The polypeptide chain of trypsinogen is hydrolyzed only after an -(Asp)4-Lys- sequence. The enzyme is inhibited by soybean trypsin inhibitor. Enterokinase is typically used in protein modification and amino acid sequence determination. |
| Unit Definition【单位定义】 | 在 pH 5.6 和 25℃ 下，一单位每分钟可将胰蛋白酶原转化为 1.0nmol 的胰蛋白酶。 |
| Physical form【外形】 | 含有磷酸钠缓冲盐的冻干粉 |
产品性质
| form【形式】 | lyophilized powder |
| Quality Level【质量水平】 | 200 |
| Specific Activity | ≥100 units/mg protein |
| mol wt【分子量】 | 150 kDa |
| purified by【纯化方式】 | chromatography |
| composition【组成】 | Protein, ≥20% Lowry |
| foreign activity【异质活性】 | aminopeptidase ≤1.5% trypsin ≤1%, free |
| storage temp.【储存温度】 | −20℃ |
安全信息
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |
