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EphB2/Fc Chimera from mouse
| 产品编号： | 3081175 |
| 规格： | >90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder |
| 包装规格： | 200 μG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
EphB2/Fc Chimera from mouse
Qek2
Sek3
Tyro5
Cek5
Drt
Nuk
Hek5
ERK
基本信息
| MDL number | MFCD01867526 |
| NACRES | NA.32 |
| General description【一般描述】 | EphB2 (Epherin receptor B2) is a receptor tyrosine kinase (RTK), which belongs to a 13 member family of ephrin receptors. This family is divided into two classes A and B. EphB2 belongs to EphB group, which contains five members. The ligands to this group of receptors are named ephrin-B1-B3. It is a bidirectional functioning membrane protein. |
| Application【应用】 | EphB2/Fc Chimera from mouse has been used for the investigation of the role of ephrinB-EphB signaling in the induction and maintenance of hyperalgesia, hyperexcitability of neurons of DRG (dorsal root ganglion) and hyperexcitability and elevated synaptic plasticity of neurons of DH (dorsal horn) regions of brain. |
| Biochem/physiol Actions【生化/生理作用】 | EphB2 (Epherin receptor B2) is involved in the development and plasticity of glutamatergic synapses, and their interaction with NMDA receptor. It can also regulate the processing of acute inflammatory pain, and mechanical allodynia as a result of nerve crush. Activation of this receptor results in recruitment and activation of Src-protein kinases. These, in turn, phosphorylate and activate NMDA receptor. It regulates the function of platelets and the development of thrombus. It is also responsible for the retraction of clots, and modulates independent platelet function in a contact-independent manner. EphB2 signaling is also involved in the modulation of axonal sprouting, which is induced by lesions. Member of the Eph receptor tyrosine kinase family shown to bind ephrin-B1, ephrin-B2, and ephrin-B3; involved in pattern formation and morphogenesis, and in arterial/venous demarcation of the developing vasculature. |
| Other Notes【其他说明】 | Extracellular domain of mouse EphB2 (amino acids 1-548) fused to the C-terminal 6X histidine-tagged Fc region of human IgG1 via a polypeptide linker. |
| Physical form【外形】 | Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, pH 8.0. |
| Analysis Note【分析说明】 | The biological activity is measured by its ability to bind recombinant mouse ephrin-B2/Fc in an ELISA assay. |
产品性质
| Quality Level【质量水平】 | 100 |
| biological source【生物来源】 | mouse |
| recombinant【重组】 | expressed in NSO cells |
| Assay【测定】 | >90% (SDS-PAGE) |
| form【形式】 | lyophilized powder |
| potency【效能】 | 0.04-2.5 ng/mL |
| mol wt【分子量】 | monomer calculated mol wt 85.3 kDa 100-110 kDa by SDS-PAGE (reducing) |
| packaging【包装】 | pkg of 200 μg |
| storage condition【储存条件】 | avoid repeated freeze/thaw cycles |
| technique(s) | ligand binding assay: suitable |
| impurities【杂质】 | endotoxin, tested |
| UniProt accession no.【UniProt登记号】 | P54763 |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | mouse ... Ephb2(13844) |
安全信息
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, type N95 (US) |
