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Extract-N-AmpTM血液PCR试剂盒

Extract-N-AmpTM Blood PCR Kit
产品编号:3086627
规格:sufficient for 100 extractions, sufficient for 100 amplifications
包装规格:1 KIT
产品类别:试剂盒
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
30866271 KIT3840
产品别名

Extract-N-AmpTM血液PCR试剂盒

Extract-N-AmpTM Blood PCR Kit

基本信息
NACRES
NA.55
General description【一般描述】
The Extract-N-Amp Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.
Application【应用】
Extract-N-Amp血液PCR试剂盒已用于从基因组和干血斑中提取DNA。它已用于聚合酶链反应(PCR)。
Features and Benefits【特点和优势】
  • Efficient 8 minute prep allows greater speed and throughput
  • No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
  • Simple, 3 step procedure with no special equipment required
  • Hot start antibody included for highly specific PCR amplification of genomic DNA
  • Compatible with multiple format (single tube or 96-well plate)
  • Can be used with whole blood or blood cards
  • Extract stable at 4 ℃ for at least 6 months
Principle【原理】
只需加入Extraction Solution并在室温下孵育5分钟,即可从10 μl全血中提取出基因组DNA。向提取物中加入Neutralization Solution,以便在PCR反应前中和抑制物。随后选取一部分DNA提取物,加入专门配制的PCR ReadyMix。
Other Notes【其他说明】
更多信息,请见www.sigma-aldrich.com/extract-n-amp。
Legal Information【法律信息】
凡购买本产品,即表示授权用户使用产品说明书所指定的专利,但仅限于将所购数量的产品用于内部研究用途。我们未明确表示、暗示,或通过禁止反言形式转让任何其他专利权(如,5′ Nuclease Process专利权)。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
依照美国专利号5,338,671和5,587,287和其他地区对应的专利,许可抗体用于体外研究用途。
Extract-N-Amp is a trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC
Components【组分】
组份可单独销售
L3289 Lysis Solution for Blood
N9784 Neutralization Solution for Blood
P8115 Extract-N-Amp PCR ReadyMix for Blood
产品性质
usage【用途】
sufficient for 100 amplifications
sufficient for 100 extractions
sufficient for 100 reactions
Quality Level【质量水平】
200
feature【特点】
dNTPs included
hotstart
technique(s)
PCR: suitable
color【颜色】
colorless
web metadata keyword.default
XNAB2R
XNAB2RE
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
安全信息
Pictograms【象形图】
GHS05
Signal word【警示用语:】
Danger
Hazard Statements
H290 - H314
Precautionary Statements
P234 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 - P363
Hazard Classifications【危险分类】
Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1B
Storage Class Code【储存分类代码】
8A - Combustible, corrosive hazardous materials
WGK
WGK 3
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