Fluorescent Dopamine Neurotransmitter Ligand, labeled with Dansyl

NA.32

This fluorescent labeled catecholamine neurotransmitter analog of dopamine (Product Name: DnsylD-1), with dansyl attached by a 5-carbon spacer arm to dopamine, provides a unique pharmacological tool. DnsylD-1 is a stable, receptor selective and high affinity pharmacological ligand that can be detected by fluorescent microscopy and FLIPR devices. Unlike recently reported synthesized fluorescent neurotransmitter analogs, DnsylD-1 contains the entire native dopamine structural moiety attached with the 5-carbon polylinker to the small fluorescence label dansyl. This structural modification minimizes steric hindrance, making DnsylD-1 an effective reagent for dopamine ligand binding and transporter uptake assays at namomolar concentrations.

A large stokes shift between excitation and emission maxima (Ex:Em, 313:515 nm) of DnsylD-1 provides high signal to noise detection with minimal autofluorescence. The dansyl moiety also changes spectral characteristics on intermolecular contact, facilitating biophysical characterization of ligand-receptor interaction. DnsylD-1 is a suitable substitute for radioactive ligand.

Applications for Fluorescent Dopamine Ligand-Neurotransmitter (DnsylD-1)

DnsylD-1 has the following applicability due to the small molecular size of the dansyl fluorescent label, a long spacer arm between the dopamine structure and the fluorescent tag, stability in aqueous solution and low autofluorescence of the surrounding cellular or tissue medium.

• Histochemical imaging and quantitation of dopamine receptor expression in vivo and vitro.
• Pharmacological applications as a substitute for radioactive ligand.
• Dopamine metabolism and turnover analysis.
• Dopamine uptake analysis.
• Dopaminergic neuron detection.
• Analysis of disease conditions associated with altered dopamine synthesis, dopamine receptor expression or dopamine uptake: human disease research applicability in the following: a) Parkinson′s disease. b) Psychological disorder, including ADHD, schizophrenia and substance abuse. c) Physiological regulation of cardiovascular, renal and immune function.

100

≥98% (HPLC)

λ_ex 333 nm; λ_em 515 nm±5 nm in DMSO

suitable for fluorescence

2-8℃

13 - Non Combustible Solids

WGK 3