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产品别名
HeLa/MMTV-luc
HeLa
产品性质
| biological source【生物来源】 | human cervix |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Epithelial |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
基本信息
| Cell Line Origin【细胞系来源】 | HeLa, genetic modification, a firefly luciferase reporter gene, MMTV promoter |
| Cell Line Description【细胞系描述】 | HeLa/MMTV-luc is a HeLa cell line stably transfected with a firefly luciferase reporter gene under the control of the MMTV promoter. HeLa/MMTV-luc can be used to detect glucocorticoid mediated transcriptional activity of chemicals. |
| DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X CSF1PO: 9,10 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18 |
| Culture Medium【培养基】 | DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 16 μg/ml Blasticidin S HCL. |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 ℃. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
产品说明
| Disclaimer【免责声明】 | This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form. |
