Immunoprecipitation Vector Kit

NA.85

The Immunoprecipitation (IP) Vector Kit provides a unique method to validate protein-protein interactions in a convenient 96-well format. This system enables potential binding partners for the protein of interest to be tagged with ”FLAG and c-Myc epitopes, followed by capture and detection using the ANTI-FLAG M2 High Sensitivity Capture Plate.

The kit provides expression vectors ”pFLAG-CMV”-2 and pc-Myc-CMV-2 designed for the cloning and expression of protein interaction candidates as N-terminal FLAG and N-terminal c-Myc fusions. Control plasmids pFLAG-CMV-2-p53, pC-Myc-CMV-2-Large T antigen and pC-Myc-CMV-2-BAP supplied are for expression of positive and negative binding partners in the IP analysis. The kit also includes the MF-2 and MR-2 verification primers.

Vector Maps and Sequences

组份不可单独销售
pc-Myc-CMV-2 10 μg
pc-Myc-CMV-2-BAP 10 μg
pc-Myc-CMV-2-Large T antigen 10 μg
pFLAG-CMV™-2 10 μg
pFLAG-CMV™-2-p53 20 μg
Verification Primer-MF 2 nmol
Verification Primer-MR 2 nmol

200

for molecular biology

buffered aqueous solution

dry ice

−20℃

13 - Non Combustible Solids

WGK 3