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JumpStartTM REDTaq® DNA Polymerase

产品编号:3102321
规格:Hot-start Taq enzyme with inert dye, 10X buffer included
包装规格:50 UNITS,250 UNITS,2500 UNITS
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
310232150 UNITS570
3102321250 UNITS1100
31023212500 UNITS7970
基本信息
MDL number
MFCD00166026
NACRES
NA.55
General description【一般描述】
JumpStart REDTaq® DNA Polymerase is Sigma′s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase.
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.
Application【应用】
JumpStart REDTaq® DNA Polymerase has been used in:
  • polymerase chain reaction (PCR) for the amplification of insulin-enterotoxin ricin fusion gene (INS-RTB)
  • endothelial cells DNA derived from reverse transcribed RNA
  • leg genomic DNA from cricket flies
  • for DNA methylation analysis
  • for the amplification of mitochondrial gene by conventional PCR
Features and Benefits【特点和优势】
  • Reduces non-specific amplification
  • Increased target yield and specificity
  • Higher the amplification irrespective of the target concentration
  • Reduce set-up time and eliminate concerns associated with manual or wax hot start methods
  • Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
  • Samples can be loaded directly onto an agarose gel for electrophoresis without loading buffers or tracking dyes
  • Assembled PCR reactions can be placed at room temperature for up to 2 hours
Unit Definition【单位定义】
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 ℃.
Other Notes【其他说明】
View more detailed information on JumpStart REDTaq enzymes at www.sigma-aldrich.com/hotstart.
Legal Information【法律信息】
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of sigma-aldrich Co. LLC
REDTaq is a registered trademark of sigma-aldrich Co. LLC
产品性质
Quality Level【质量水平】
200
form【形式】
liquid
usage【用途】
sufficient for 250 reactions
sufficient for 2500 reactions
sufficient for 50 reactions
feature【特点】
dNTPs included: no
hotstart
concentration【浓度】
1 unit/μL
technique(s)
PCR: suitable
color【颜色】
red
input
purified DNA
shipped in【运输】
wet ice
storage temp.【储存温度】
−20℃
packaging【包装】
The enzyme is provided with an optimized 10× reaction buffer.
安全信息
Pictograms【象形图】
GHS07
Signal word【警示用语:】
Warning
Hazard Statements
H315 - H319 - H335
Precautionary Statements
P302 + P352 - P305 + P351 + P338
Hazard Classifications【危险分类】
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs【靶器官】
Respiratory system
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
WGK 3
Personal Protective Equipment【个人防护装备】
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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