JumpStart^TM Taq DNA聚合酶

MFCD00166026

NA.55

The Taq DNA polymerase activity is inactivated by combining the enzyme with JumpStart^™ Taq antibody, a neutralizing monoclonal antibody to Taq DNA polymerase. Antibody inactivation provides a simple, efficient procedure for hot-start polymerase chain reaction (PCR). Hot start PCR can significantly improve the results of DNA amplification by reducing the generation of nonspecific amplification products and primer-dimer artifacts. When used in PCR, JumpStart Taq DNA polymerase is inactive at low (room) temperature. When the temperature is raised above 70 ℃ in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active.

JumpStart^™ Taq DNA polymerase has been used:

• In the PCR amplification of DNA isolated from herbarium specimens.
• In the PCR reaction mixture for randomly amplified polymorphic DNA polymerase chain reaction (RAPD PCR).
• To amplify and detect a point mutation in the EGFR exon 19 using specific cancer cell lines.
• In real-time quantitative PCR
• For PCR amplification of complex genomic or cDNA templates
• For the PCR amplification of very low-copy number targets
• For the PCR amplification of many thermal cycles (>35), and multiple primer pairs in the same reaction tube

• 用于需要降低非特异性扩增的PCR扩增
• 用于多重PCR
• 用于降低引物二聚体

Sigma的JumpStart Taq DNA聚合酶是一种抗体失活的热启动酶，设计用于最大限度降低非特异性扩增并同时提高靶标的产量。 一旦反应温度达到 70℃，Taq DNA聚合酶活性就会得到恢复，使得PCR具有更高的特异性和产量。这种抗体-酶复合物可实现简单便捷的设置，并相对于手动热启动技术具有更低的污染风险。 该酶还可以被加入到预混液制备中，从而实现反应之间更高的一致性。
可访问www.sigma-aldrich.com/hotstart查看更多关于JumpStart Taq酶的详细信息。

在74 ℃，一单位的酶可以在30分钟内将10 nmol的总dNTP插入至可酸沉淀的DNA中。

本产品的使用受到如下一项或多项美国专利及其对应的美国境外专利声明保护：US 8,404,464及US 7,972,828. 购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可。

抗体授权用于在美国专利5,338,671和5,587,287及其在其他国家相应专利保护下的体外研究用途。
JumpStart is a trademark of sigma-aldrich Co. LLC

300

liquid

sufficient for 1500 reactions
sufficient for 250 reactions
sufficient for 50 reactions

dNTPs included: no
hotstart

2.5 units/μL

PCR: suitable

colorless

purified DNA

agriculture

wet ice

−20℃

按不含 MgCl₂的10x反应缓冲液形式提供。 包含单独一管的25 mM MgCl₂
JumpStart Taq DNA聚合酶是以10x反应缓冲液形式提供的，并可选择是否含有MgCl_2。 不含镁的10x缓冲液还包含单独一管的25 mM MgCl₂用于优化。

10 - Combustible liquids

WGK 3