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产品性质
| biological source【生物来源】 | human unknown/unspecified |
| growth mode【生长模式】 | Suspension |
| karyotype【核型】 | 46,XX,-8,+t(3;8)(p12-13;q12),t(11;14)(q13;q32) |
| morphology【形态学】 | Lymphoblastoid |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Human Caucasian B-prolymphocytic leukaemia, EBV-transformed |
| Cell Line Description【细胞系描述】 | JVM-2 has been derived from EBV and TPA treated mononuclear cells from a 63 year old female patient with B-prolymphocytic leukaemia. Thirty-three percent of cells were shown to be positive for surface lambda light chain, 37% for IgA, 29% for IgG and 3% for IgM and IgD. In addition, 50% were found to express cytoplasmic IgM and lambda light chain. Expression of the following leukocyte differentiation antigens was found: CD9, CD19, CD20, CD23, CD24, MA6, CD37, CD38, FMC1, FMC7, MHC Class I, FMC16, MHC Class II DR/DP/DQ. The immunoglobulin heavy-and light-chain genes showed the same pattern of rearrangement as in the original prolymphocytes. The cells are lymphoblastoid with large floating aggregates present. The cell line has been eradicated from mycoplasma at ECACC. |
| Application【应用】 | Ig expression of neoplastic cells |
| DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X CSF1PO: 11 D13S317: 11,13 D16S539: 12,13 D5S818: 11,12 D7S820: 10,11 THO1: 6,9 TPOX: 8,11 vWA: 17 |
| Culture Medium【培养基】 | RPMI 1640 +2mM Glutamine + 10% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Maintain cultures between 3-9 x 100,000/ml; 5% CO2; 37℃. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
