Lambda DNA Mixed Digest

NA.25

Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Lambda DNA Mixed Digest has been used for DNA electrophoresis.
Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

10mM Tris-HCl 中的溶液，pH 8.0，1mM EDTA

Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.

For optimal resolution, the recommended agarose gel concentration is 0.4%.

200
100

buffered aqueous solution

100 uses (0.1 mg size)
20 uses (20 μg size)

suitable for electrophoresis (DNA)

−20℃

12 - Non Combustible Liquids