LuminoCt^® SYBR^® Green qPCR ReadyMix^TM

NA.55

LuminoCt SYBR Green qPCR ReadyMix is designed to deliver unsurpassed assay speeds without sacrificing accuracy, precision, or sensitivity. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers. Inclusion of JumpStart^™ Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.

• Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms
• ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays
• Kit is not compatible with qPCR instruments that utilize glass capillary tubes

LuminoCt^® SYBR^® Green qPCR ReadyMix^™ has been used for fast qPCR and quantitative RT-PCR amplifications.

Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of sigma-aldrich Co. LLC
LuminoCt is a registered trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
SabreLite is a registered trademark of Pelican Products, Inc.

组份可单独销售
LuminoCt SYBR Green qPCR ReadyMix 2 X
R4526 100X ROX internal reference dye

200

liquid

sufficient for 100 reactions
sufficient for 2000 reactions
sufficient for 500 reactions

dNTPs included
hotstart

qPCR: suitable

colorless

purified DNA

agriculture

for use with ABI 5700
for use with ABI 7000
for use with ABI 7300
for use with ABI 7500 Fast
for use with ABI 7500
for use with ABI 7700
for use with ABI 7900 Fast
for use with ABI 7900 HT
for use with ABI 7900
for use with ABI StepOne
for use with ABI StepOnePlus
for use with ABI ViiA 7
for use with Bio-Rad CFX384
for use with Bio-Rad CFX96
for use with Bio-Rad MJ Chromo4
for use with Bio-Rad MJ Opticon 2
for use with Bio-Rad MJ Opticon Cepheid SmartCycler
for use with Bio-Rad MJ Opticon
for use with Bio-Rad MiniOpticon
for use with Eppendorf^® Mastercycler ep realplex2 s
for use with Eppendorf^® Mastercycler ep realplex
for use with Illumina Eco qPCR
for use with Qiagen Corbett Rotor-Gene 3000
for use with Qiagen Corbett Rotor-Gene 6000
for use with Qiagen Corbett Rotor-Gene Q
for use with Roche LightCycler 480
for use with Strategene Mx3000P
for use with Strategene Mx3005P
for use with Strategene Mx4000

SYBR^® Green

wet ice

−20℃

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL

10 - Combustible liquids

WGK 1