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产品性质
| biological source【生物来源】 | human skin |
| growth mode【生长模式】 | Adherent |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Fibroblast |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Human skin fibroblast |
| Cell Line Description【细胞系描述】 | A thioguanine resistant derivative of MRC-5 CV1, selected with 5μg/ml 6-thioguanine. |
| Application【应用】 | Genetic studies |
| DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X,Y CSF1PO: 11,12 D13S317: 11,14 D16S539: 11 D5S818: 11,12 D7S820: 10,11 THO1: 8 TPOX: 8 vWA: 15 |
| Culture Medium【培养基】 | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS). |
| Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm3 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
