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产品性质
| biological source【生物来源】 | human blood |
| growth mode【生长模式】 | Suspension |
| karyotype【核型】 | Not specified |
| morphology【形态学】 | Lymphoblastoid |
| products【产品】 | Not specified |
| receptors【受体】 | Not specified |
| technique(s) | cell culture | mammalian: suitable |
| relevant disease(s)【相关疾病】 | cancer |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −196℃ |
基本信息
| Cell Line Origin【细胞系来源】 | Human acute myeloblastic leukaemia |
| Cell Line Description【细胞系描述】 | ML-1 is one of three celll lines isolated in 1978 from the peripheral blood of a 24 year old male patient with acute myeloblastic leukaemia. The cells can convert to more mature cells by the use of DMSO. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis. |
| Application【应用】 | ML-1 has been used to compare the cell response to mafosfamide cyclohexylamine salt, 4-hydro-peroxy-cyclophosphamide and β-D-glucose-isophosphoramide mustard, which are oxazaphosphorine agents. |
| DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X CSF1PO: 10,11 D13S317: 9,12 D16S539: 9,12 D5S818: 12 D7S820: 9,11 THO1: 7,9.3 TPOX: 8,10 vWA: 16 |
| Culture Medium【培养基】 | RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS (F2442) |
| Subculture Routine【传代培养常规】 | Maintain cultures between 2-9 x100,000 cells/ml; 5% CO2; 37℃. Freeze in 10% DMSO + 90% FBS. Immediately after resuscitation, pellet cells by centrifugation at 150 x g for 5 minutes and resuspend the cell pellet in fresh medium. This is to remove the presence of DMSO which may cause differentiation of the cells if allowed to remain. |
| Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |
