Anti-Actin, Muscle specific antibody, Mouse monoclonal

NA.41

Monoclonal Anti-Actin, muscle specific (mouse IgG1 isotype) is derived from the hybridoma HHF-35 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with SDS extracted protein fraction of human myocardium. The two major cytoskeletal proteins implicated in cell motility are actin and myosin. Although actin is one of the most conserved eukaryotic proteins, it is expressed in mammals and birds as six isoforms characterized by electrophoretic mobility and amino acid sequence analysis.

SDS-extracted protein fraction of human myocardium.

The antibody may be used in various immunochemical techniques including, immunoblotting, immunocytochemistry and immunohistochemistry.

Actin and myosin are constituents of many cells types and are involved in a myriad of cellular processes including locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

mouse

unconjugated

purified from hybridoma cell culture

primary antibodies

HHF-35, monoclonal

buffered aqueous solution

antigen ~42 kDa

monkey, bovine, mouse, rat, canine, chicken, hamster, human

~1.0 mg/mL

immunocytochemistry: 2-4 μg/mL using A10 cells
immunohistochemistry: suitable
western blot: 0.1-0.2 μg/mL using A10 cell extracts

IgG1

dry ice

−20℃

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids