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Anti-AKT1S1 antibody, Mouse monoclonal
| 产品编号： | 3140731 |
| 规格： | clone AKT1S1-3, purified from hybridoma cell culture |
| 包装规格： | 200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-AKT1S1 antibody, Mouse monoclonal
Monoclonal Anti-AKT1 substrate 1 (proline-rich)
Monoclonal Anti-PROLINE-RICH AKT SUBSTRATE, 40-KD
Monoclonal Anti-Lobe
Monoclonal Anti-PRAS40
基本信息
| NACRES | NA.44 |
| General description【一般描述】 | Monoclonal Anti-AKT1S1 (mouse IgG1 isotype) is derived from the hybridoma AKT1S1-3 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to the N-terminus of human AKT1S1. Akt1 substrate 1 (AKT1S1) is also named as proline-rich Akt-substrate of 40 kDa (PRAS40). The rapamycin-sensitive mammalian target of rapamycin complex 1 (mTORC1) consists of mTOR, raptor, mLST8 and AKT1S1, that controls protein translation. AKT1S1 contains a TOR signaling (TOS) motif that mediates its binding to mTORC1. AKT1S1 is a raptor-associated protein that interacts with mTORC1 and subsequently inhibits the mTORC1 signaling pathway. This raptor-binding protein also inhibits phosphorylation of S6K1 and cell growth. Expression of AKT1S1 has been implicated in breast and pulmonary carcinogenesis, as well as in mediating insulin sensitivity . Monoclonal Anti-AKT1S1 antibody is specific for human AKT1S1 (approx. 40 kDa). |
| Immunogen【免疫原】 | fusion protein corresponding to the N-terminus of human AKT1S1. The corresponding sequence differs by a single amino acid in rat, and by 2 amino acids in mouse AKT1S1. |
| Application【应用】 | Monoclonal Anti-AKT1S1 antibody is suitable for use in western blot (2-8 μg/mL using whole extracts of human MDA-MB-231cells) and immunoprecipitation. |
| Biochem/physiol Actions【生化/生理作用】 | Akt1 substrate 1 (AKT1S1) is involved in the PI3KAkt/ protein kinase B (PKB) survival pathway. Phosphorylation of AKT1S1 by Akt and mammalian target of rapamycin complex 1 (mTORC1) disrupts the binding between mTORC1 and AKT1S1, relieves the inhibitory effect of AKT1S1 on mTORC1 activity, and leads to the binding of AKT1S1 to 14-3-3, a cytosolic anchor protein. The binding of AKT1S1 to 14-3-3 requires amino acids and insulin, which is partially inhibited by rapamycin. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone【克隆】 | AKT1S1-3, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~40 kDa |
| species reactivity | human |
| concentration【浓度】 | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): suitable western blot: 2-8 μg/mL using whole extracts of human MDA-MB-231cells. |
| isotype【同位素/亚型】 | IgG1 |
| UniProt accession no.【UniProt登记号】 | Q96B36 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... AKT1S1(84335) |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
