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Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) antibody produced in mouse
产品别名
Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) antibody produced in mouse
Monoclonal Anti-Phosphatase, Alkaline, bacterial
基本信息
| MDL number | MFCD02095875 |
| NACRES | NA.46 |
| General description【一般描述】 | Bacterial alkaline phosphatase (BAP) is a periplasmic enzyme that is encoded by the phoA gene. The use of a BAP tag to the target gene yields a stable fusion product that does not affect the bioactivity and biodistribution of the target gene in recombinant expression vector systems. Hence, anti-BAP antibodies can be useful tools for the detection and analysis of target biomolecules fused to BAP tags.Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) (mouse IgG1 isotype) is derived from the BAP-77 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a BAP tagged fusion protein. Bacterial alkaline phosphatase (BAP) also known as PhoA, EC 3.1.3.1 is an 50 kDa protein, derived from E. coli. |
| Immunogen【免疫原】 | A bacterial alkaline phosphatase (BAP) tagged fusion protein. |
| Application【应用】 | Monoclonal Anti-Bacterial Alkaline Phosphatase (BAP, PhoA) antibody is suitable for use in western blot (at 1:20,000 dilutions using purified Escherichia coli BAP) and indirect ELISA. |
| Biochem/physiol Actions【生化/生理作用】 | Bacterial alkaline phosphatase (BAP) enzyme regulates the catabolism of phosphate esters. Alkaline phosphatase appears to require export to the periplasm to show enzymatic activity. Fusions of the secreted alkaline phosphatase to an integral cytoplasmic membrane protein of E. coli shows different activities depending on where with the membrane protein the alkaline phosphatase is fused. Fusions to positions in or near the periplasmic (extracellular) domain lead to high alkaline phosphatase activity, whereas those to positions in the cytoplasmic domain give low activity. |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | alkaline phosphatase conjugate |
| antibody form【抗体形式】 | ascites fluid |
| antibody product type | primary antibodies |
| clone【克隆】 | BAP-77, monoclonal |
| mol wt【分子量】 | antigen ~50 kDa |
| species reactivity | bacteria |
| technique(s) | indirect ELISA: suitable western blot: 1:20,000 using purified Escherichia coli BAP |
| isotype【同位素/亚型】 | IgG1 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
