Monoclonal Anti-Bcl-2 antibody produced in mouse

MFCD00239644

NA.41

BCL2 (B-cell lymphoma 2) consists of pro- and antiapoptotic functions. It belongs to BCL2 family of proteins. It is mapped to human chromosome 18q21.3.

synthetic peptide corresponding to residues 41-54 of the Bcl-2 protein, conjugated to thyroglobulin.

Monoclonal Anti-Bcl-2 antibody produced in mouse is suitable for western blotting at a concentration of 1:1,000 by using human HeLa cell extract. It is also suitable for immunohistochemistry by using formalin-fixed, paraffin-embedded tissue sections or frozen sections. Further, the antibody may be used for the localization of Bcl-2, by implementing numerous assays like- microarray, electron microscopy and immunoprecipitation.
Anti-BCL2 (Ab-56) antibody has been used in western blotting and immunocytochemistry.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Protooncogene BCL2 encodes a 26 kD integral outer mitochondrial membrane protein expressed in sub-cellular components such as the mitochondria and nucleus. While mitochondrial BCL2 inhibits apoptosis, BCL2 present in the nucleus may activate cell death. It may also regulate autophagy during starvation. Additionally, BCL2 facilitates the survival of central and peripheral neurons grown in culture in the absence of neurotrophic factors.
BCL2 (B-cell lymphoma 2) plays an important role in the growth and progression of cancer. Overexpression of BCL2 (B-cell lymphoma 2) results in poor clinical predictions in diffuse large B-cell lymphoma (DLBCL). Bcl2 is highly essential for the survival of kidney and melanocyte stem cells and mature lymphocytes. It may control Bcl2 multiple initiator caspases.

200

mouse

unconjugated

ascites fluid

primary antibodies

Bcl-2-100, monoclonal

antigen 26 kDa

15 mM sodium azide

human

mouse

electron microscopy: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:1,000 using human HeLa cell extract

IgG1

P10415

dry ice

−20℃

human ... BCL2(596)

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

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