Anti-Calnexin antibody, Mouse monoclonal

MFCD01094399

Anti-Calnexin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma TO-5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Calnexin contains a long (461 amino acids) N-terminal Ca²⁺-binding domain extending into the lumen of the endoplasmic reticulum (ER), a short (22 amino acids) trans-membrane segment and an acidic cytosolic domain (96 amino acids).
Calnexin (p88, IP90) is a calcium-binding, type I integral membrane protein, localized primarily in the endoplasmic reticulum (ER). Calnexin binds newly synthesized glycoproteins and misfolded proteins and is believed to play a critical role in quality control processes during protein synthesis and folding. Calnexin acts as a lectin like chaperone that binds oligosaccharide residues of newly synthesized N-linked glycoproteins. The lectin specificity of calnexin and its soluble homologue calreticulin has been identified as high mannose oligosaccharides terminating in monoglucosyl residues linked through α1-3. Calnexin has been shown to be associated with several cell surface proteins, including MHC class I heavy chain, T-cell receptor (TCR), and B cell membrane immunoglobulin during translocation through the ER. It also forms complexes with other resident ER proteins involved in Ca²⁺-dependent retention of proteins.

Mouse monoclonal clone TO-5 anti-Calnexin antibody recognizes human Calnexin, ~90 kDa.

Anti-Calnexin antibody, Mouse monoclonal has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• flow cytometry
• immunocytochemistry
• immunohistochemistry
• immunofluorescence analysis

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

200

mouse

unconjugated

purified immunoglobulin

primary antibodies

TO-5, monoclonal

buffered aqueous solution

antigen ~90 kDa

human

~2 mg/mL

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 0.5-1.0 μg/mL using total cell extract of HeLa cells.

IgG1

P27824

dry ice

−20℃

human ... CANX(821)

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12 - Non Combustible Liquids

WGK 3