Monoclonal Anti-Calreticulin antibody produced in mouse

NA.44

Monoclonal Anti-Calreticulin (mouse IgG1 isotype) is derived from the hybridoma TO-11 produced by the fusion of mouse myeloma cells (P3-X63-AG8.653 cells) and splenocytes from BALB/c mice. Calreticulin is a molecular chaperones in endoplasmic reticulum lumen. Calreticulin acts as a lectin-like chaperone binding oligosaccharide residues of newly synthesized N-linked glycoproteins, and misfolded proteins. Calreticulin is a protein binding Ca²⁺ ions. It is encoded by the CALR gene in humans.
Monoclonal Anti-Calreticulin (mouse IgG1 isotype) is derived from the hybridoma TO-11 produced by the fusion of mouse myeloma cells (P3-X63-AG8.653 cells) and splenocytes from BALB/c mice.

Monoclonal Anti-Calreticulin antibody produced in mouse is suitable for the following applications:

• Flow cytometry
• Immunocytochemistry
• Immunohistochemistry
• Indirect ELISA
• Western blotting

It is believed to play a critical role in quality control processes during protein synthesis and folding. Increased expression of calreticulin increases Ca²⁺ storage capacity of the ER. It also appears to modulate store-operated Ca²⁺ -influx, and to alter Ca²⁺ transport by the sarcoplasmic/ER Ca²⁺ -ATPase (SERCA). Overexpression of calreticulin results in increased sensitivity of HeLa cells to drug-induced apoptosis. In contrast, calreticulin-deficient cells show increased resistance to apoptosis. Calreticulin gene disruption leads toimpairment of cardiac development. Loss of calreticulin function favors ubiquitin-proteosome activity. It plays a critical role in Ca²⁺ homeostasis.
Calreticulin and Its variants causes differential effects on thrombopoiesis. Somatic mutations of calreticulin (CALR) gene causes thrombocythemia (ET) and primary myelofibrosis (PMF).

Culture supernatant solution containing 15 mM sodium azide.

200

mouse

unconjugated

tissue culture supernatant

primary antibodies

TO-11, monoclonal

buffered aqueous solution

antigen ~50 kDa

human

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect ELISA: suitable
western blot: 1:50-1:100 using total cell extract of HeLa cells

IgG1

P27797

dry ice

−20℃

human ... CALR(811)

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12 - Non Combustible Liquids

WGK 3

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)