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Anti-Caspase 9 antibody,Mouse monoclonal

产品编号:3142166
规格:clone CAS9, purified from hybridoma cell culture
包装规格:0.2 ML
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
31421660.2 ML5530
基本信息
MDL number
MFCD01864176
NACRES
NA.41
General description【一般描述】
Monoclonal Anti-Caspase 9 (mouse IgG1 isotype) is derived from the CAS9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to amino acids of human caspase 9 protein, conjugated to keyhole limpet hemocyanin (KLH). Caspases (cysteine-requiring aspartate protease) are a family of proteases that share similarities in amino acid sequences, structure and substrate specificity. Caspases are normally present in the cell as inactive procaspases. Caspase 9 (also known as Mch6 and ICE-LAP6) gene encodes a protein product of 37 kDa (reported also as 46 kDa), which undergoes a cleavage upon activation, leading to p18/p15/p9 subunits. It is a member of the CED-3 subfamily, bearing high similarity to caspase. High level expression of caspase 9 is found in the heart, testis and ovary.
Immunogen【免疫原】
synthetic peptide corresponding to amino acids 299-316 of human caspase 9 protein conjugated to KLH.
Application【应用】
Anti-Caspase 9 antibody,Mouse monoclonal has been used in:
  • western blot assay
  • immunocytochemistry
  • enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions【生化/生理作用】
Caspase 9 is a downstream protease, activated in caspase 3- and granzyme B-mediated apoptosis. Procaspase 9 is activated when a complex is formed with the cofactor apoptotic protease activating factor-1 (Apaf-1) through the caspase recruitment domain (CARD). Caspase 9 plays a critical role in the survival of neuronal progenitors within the embryonic forebrain and cortex. Its deficiency leads to defective brain development and embryonic lethality. Overexpression of caspase 9, but not of a mutant in which the catalytic Cys was replaced with an Ala, induces apoptosis in MCF7 cells.
Physical form【外形】
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
产品性质
Quality Level【质量水平】
200
biological source【生物来源】
mouse
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
purified from hybridoma cell culture
antibody product type
primary antibodies
clone【克隆】
CAS9, monoclonal
form【形式】
buffered aqueous solution
mol wt【分子量】
antigen 37 kDa
species reactivity
human
concentration【浓度】
~2 mg/mL
technique(s)
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using a purified recombinant human caspase 9 preparation
isotype【同位素/亚型】
IgG1
UniProt accession no.【UniProt登记号】
P55211
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
human ... CASP9(842)
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
10 - Combustible liquids
WGK
nwg
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