Anti-Cdh1 antibody,Mouse monoclonal

MFCD03457549

NA.41

Monoclonal Anti-Cdh1 (mouse IgG1 isotype) is derived from the DCS-266 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human cadherin-1 (Cdh1).
Cdh1 homophilic major cell adhesion molecule in epithelial cells belongs to cadherin superfamily.

Monoclonal Anti-Cdh1 reacts specifically with human Cdh1.

recombinant human Cdh1.

Monoclonal Anti-Cdh1 antibody was used for immunoblotting of lysates in a study of Identification of Novel Human Cdt1-binding Proteins. It is suitable for western blotting with 2-4 μg/mL by using a HeLa cell nuclear extract. It is also suitable for immunoprecipitation and microarray.

Monoclonal Anti-Cdh1 reacts specifically with human Cdh1. In eukaryotes, regulation of cell cycle progression depends on the expression of cyclins proteins. Degradation of mitotic cyclins follows ubiquitin-dependent pathways. The anaphase-promoting complex/cyclosome (APC/C) plays a vital role in progression. Initially at anaphase the APC/C requires the activator protein CDC20 to target securin and cyclin B1 for proteasome-dependent degradation, but later it require Cdh1 to maintain its active state till the next S phase. Another cyclin A/cdk2 mediated phosphorylation of Cdh1 may also prevents the activatory assembly of Cdh1 with APC, thus creating an environment permissive for accumulation of APC targets. It mediates Ca²⁺ dependent homophilic interactions, as the major adhesion receptor in adherens junctions.

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.

200

mouse

unconjugated

purified from hybridoma cell culture

primary antibodies

DCS-266, monoclonal

buffered aqueous solution

antigen 50 kDa

human

~2 mg/mL

immunoprecipitation (IP): suitable
microarray: suitable
western blot: 2-4 μg/mL using a HeLa cell nuclear extract

IgG1

Q9UM11

dry ice

−20℃

human ... CDH1(999)

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