Monoclonal Anti-DDB1 antibody produced in mouse

NA.41

Synthetic peptide congugated to protein carrier. The mice were immunized with a combination of N & C peptide. The Swiss Prot ID is Q16531.

For ELISA: Use at an assay dependent dilution. For WB(ECL): Use at a dilution of 1:1,000 in 5% Milk/PBS containing 0.05% Tween. We recommend an overnight incubation at 4¡C. Secondary antibody (GTX30590) 2 hours at RT with dilution of 1:5,000 in 5% Milk/PBS containing 0.05% Tween. Not tested in other applictions. Optiamal dilutions/concentrations should be determined by the researcher.

The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells. The injected DDB protein stimulated DNA repair to normal levels in those strains that lacked the DDB activity but did not stimulate repair in cells from XPE patients that contained the activity. These results provided direct evidence that defective DDB activity causes the repair defect in a subset of XPE patients and establishes a role for this activity in nucleotide-excision repair in vivo. It remains for mutation analysis to demonstrate whether the defect in XPE patients is in the DDB1 gene or the DDB2 gene.

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Phosphate-buffered saline, pH 7.2, containing no preservatives

100

mouse

unconjugated

purified immunoglobulin

primary antibodies

1322
monoclonal

liquid

130 kDa

human

3 mg/mL

ELISA: suitable
western blot: 1:1000

IgG

Q16531

wet ice

−20℃

human ... DDB1(1642)

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12 - Non Combustible Liquids