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Monoclonal Anti-DICER1 antibody produced in mouse
产品编号: | 3144122 |
规格: | ~1.0 mg/mL, clone DCR1, purified immunoglobulin, buffered aqueous solution |
包装规格: | 200 μL |
产品类别: | 进口试剂 |
品牌: | Sigma-Aldrich |
优惠价: | 立即咨询 |
产品别名
Monoclonal Anti-DICER1 antibody produced in mouse
Anti-DCR1
Anti-HERNA
Anti-Helicase-MOI
Anti-Helicase with RNASE motif
Anti-K12H4.8-LIKE
基本信息
NACRES | NA.41 |
General description【一般描述】 | Dicer is a member of the RNase III family containing an N-terminal DEXH-box RNA helicase/ATPase domain, followed by a domain of unknown function (DUF283), a PAZ domain, which anchors the 3′-end of the guided siRNA, two RNase III domains, and a dsRBD. The current model for dsRNA cleavage by Dicer predicts that the two ribonuclease III domains of Dicer dimerize to form the catalytic center that is responsible for cleaving long dsRNA in co-operation with two RNA binding domains, PAZ and dsRNA-binding domain. Monoclonal Anti-DICER1 (mouse IgG1 isotype) is derived from the hybridoma DCR1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a fusion protein corresponding to a fragment of human DICER1. |
Application【应用】 | The antibody may be used in several immunological techniques including immunoblotting and real‐ ime PCR. |
Biochem/physiol Actions【生化/生理作用】 | Dicer catalyzes the first step in the RNA interference (RNAi) pathway and initiates formation of the RNA-induced silencing complex (RISC). The dsRNA is processed into small fragments called short interfering RNA (siRNA) or microRNA (miRNA) of typically 21-25 nucleotides long with a two-base overhang on the 3′ end. SiRNAs and miRNAs serve as guide to direct the RNA-induced silencing complex (RISC) to complementary RNAs to degrade them or prevent their translation. DICER1-deficient colorectal cancer cells have an enhanced ability to initiate tumors and metastasis. Dicer functions as an indirect tumor suppressor, as silencing Dicer expression makes cancer cells more malignant. |
Physical form【外形】 | 0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。 |
产品性质
Quality Level【质量水平】 | 200 |
biological source【生物来源】 | mouse |
conjugate【偶联物】 | unconjugated |
antibody form【抗体形式】 | purified immunoglobulin |
antibody product type | primary antibodies |
clone【克隆】 | DCR1, monoclonal |
form【形式】 | buffered aqueous solution |
mol wt【分子量】 | antigen ~218 kDa |
species reactivity | human, mouse, rat |
concentration【浓度】 | ~1.0 mg/mL |
technique(s) | western blot: 2.5-5.0 μg/mL using whole extract of human HEK-293T cells |
isotype【同位素/亚型】 | IgG1 |
UniProt accession no.【UniProt登记号】 | Q9UPY3 |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −20℃ |
Gene Information | human ... DICER1(23405) mouse ... Dicer1(192119) rat ... Dicer1(299284) |
产品说明
Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |