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Anti-EDEM1 antibody,Mouse monoclonal
| 产品编号： | 3144433 |
| 规格： | clone EDEM1-17, purified from hybridoma cell culture |
| 包装规格： | 100 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
Anti-EDEM1 antibody,Mouse monoclonal
Anti-ER degradation enhancer, mannosidase alpha-like 1
Anti-EDEM
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| clone【克隆】 | EDEM1-17, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen 75/65 kDa |
| species reactivity | human, mouse |
| concentration【浓度】 | ~1.0 mg/mL |
| technique(s) | immunoprecipitation (IP): suitable western blot: 1-2 μg/mL using whole extracts of HEK-293T cells expressing recombinant human or mouse EDEM1 |
| isotype【同位素/亚型】 | IgG2a |
| UniProt accession no.【UniProt登记号】 | Q92611 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... EDEM1(9695) |
基本信息
| General description【一般描述】 | Monoclonal Anti-EDEM1 (mouse IgG2a isotype) is derived from the hybridoma EDEM1-17 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human EDEM1. conjugated to keyhole limpet hemocyanin (KLH). EDEM1 (ER degradation-enhancing alphamannosidase-like protein 1) is a putative mannose binding lectin. In mammalian cells, EDEM1 is localized to the endoplasmic reticulum (ER), mainly as a soluble glycoprotein. |
| Application【应用】 | Monoclonal Anti-EDEM1 antibody produced in mouse has been used in:
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| Biochem/physiol Actions【生化/生理作用】 | EDEM1 (ER degradation-enhancing alphamannosidase-like protein 1) has the ability to interact with the COOH-terminus of calnexin. It lacks mannosidase activity. ER degradation-enhancing α-mannosidase-like 1 is an enzyme encoded by the EDEM1 gene in humans. It is a chaperone for rod opsin and its expression can be used to promote correct folding enhancing degradation of mutant proteins in the ER to combat protein-misfolding disease. The endogenous EDEM1 in cells that are not stressed by the expression of a transgenic misfolded protein reaches the cytosol and is degraded by basal autophagy. EDEM may maintain the retrotranslocation competence of NHK by inhibiting aggregation. It is also considered responsible for directing aberrant proteins for ERAD (ER-associated protein degradation). |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | nwg |
