Anti-GAD65 antibody , Mouse monoclonal

MFCD03455043

NA.41

Glutamate decarboxylase 2 (GAD2) encodes GAD65, an isoform of glutamate decarboxylase. GAD65 is majorly expressed in axon terminal regions in neurons. GAD65 is present in the synaptic vesicles. GAD2 is mapped to human chromosome 10p12.1. It has an N-terminal pyridoxal 5′-phosphate (PLP) binding −domain.

Monoclonal Anti-GAD65 antibody produced in mouse has been used in the detection of recombinant GAD65 using ELISA.

Glutamate decarboxylase 2 (GAD2) protein GAD65 catalyzes the formation of γ-aminobutyric acid (GABA) from glutamic acid. Palmitoylation of GAD65 is crucial for protein sorting in synaptic clusters. GAD65 autoantigen is prevalent in type 1 diabetes. Its expression increases on allergen exposure. GAD65, along with γ-aminobutyric acid (GABA), may play a key role in the pathogenesis of asthma.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

200

mouse

unconjugated

purified from hybridoma cell culture

primary antibodies

N-GAD65, monoclonal

buffered aqueous solution

antigen ~65 kDa

rat, monkey, human, mouse

~1.0 mg/mL

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.25-0.5 μg/mL using whole extracts of rat brain

IgG1

Q05329

dry ice

−20℃

human ... GAD2(2572)
mouse ... Gad2(14417)
rat ... Gad2(24380)

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 1