Monoclonal Anti-hnRNP-K/J antibody produced in mouse

MFCD06798704

NA.41

Monoclonal Anti-hnRNP-K/J (mouse IgG2b isotype) is derived from the 3C2 hybridoma produced by the fusion of mouse myeloma cells (SP2/0 cells) and splenocytes from BALB/c mice immunized with hnRNP′s purified by oligo (dC) affinity chromatography.

hnRNPs purified by oligo (dC) affinity chromatography.

The antibody may be used in various immunochemical techniques including enzyme linked immunosorbent assay (ELISA), immunoblotting, chromatin immunoprecipitation (ChIP), immunocytochemistry, antibody-based supershift assay and electrophoretic mobility shift assay (EMSA).

Heterogeneous nuclear ribonucleoproteins (hnRNPs) act in several biological activities such as transcription, pre- mRNA processing, cytoplasmic mRNA translation and turnover. hnRNP-K is found both in the nucleus, cytoplasm, and mitochondria, therefore, it was found to be involved in many biological events in the cell like: transcription, chromatin remodeling, splicing and translation. hnRNP-K is regulated by different signaling cascades and can bind kinases. As a consequence, it can mediate signals from the cytoplasm to the nucleus.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

200

mouse

unconjugated

purified immunoglobulin

primary antibodies

3C2, monoclonal

buffered aqueous solution

antigen ~60 kDa

bovine, Xenopus, hamster, human

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL using total cell extract of MBDK cells

IgG2b

P61978

dry ice

−20℃

human ... HNRNPK(3190)

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12 - Non Combustible Liquids

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