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单克隆抗-MDM2 小鼠抗
Monoclonal Anti-MDM2 antibody produced in mouse
| 产品编号： | 3148293 |
| 规格： | ~2 mg/mL, clone MD-219, purified immunoglobulin, buffered aqueous solution |
| 包装规格： | 0.2 ML |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
单克隆抗-MDM2 小鼠抗
Monoclonal Anti-MDM2 antibody produced in mouse
基本信息
| MDL number | MFCD03792424 |
| NACRES | NA.41 |
| General description【一般描述】 | Mouse 3T3 cell double minute 2 (MDM2) gene was originally found on mouse double minute chromosomes. Transformed mouse 3T3 cell double minute 2 (MDM2) is an E3 ligase expressed in podocytes. It is overexpressed in lymphomas. |
| Specificity【特异性】 | The antibody shows predominantly nuclear and some cytoplasmic staining in cells. |
| Immunogen【免疫原】 | recombinant mouse MDM2. |
| Biochem/physiol Actions【生化/生理作用】 | In addition, to regulation MDM2 also mediates the degradation of p53. MDM2 protein has been shown to activate cell proliferation by stimulating the S-phase-inducing transcription factors, E2F1/DP1. Overexpression of MDM2 results in reduced quantities of co-expressed p53, and disruption of the p53-MDM2 interaction by mutation results in both activation and accumulation of p53. The amplification of the MDM2 gene has been demonstrated in various human neoplasms. MDM2 is frequently overexpressed in the nuclei of soft-tissue sarcomas, and to a much lesser extent in a wide variety of malignancies, such as bladder, breast, testes, and cervix cancers, and in glial cells, and a range of hematological disorders. Monoclonal antibody reacting specifically with MDM2 is an essential tool in defining the interactions and distributions of MDM2, its function in signaling pathways and in mutual regulation of p53. Transformed mouse 3T3 cell double minute 2 (MDM2) acts as a negative regulator of the tumor suppressor p53. It also regulates the cell cycle and inflammatory activities. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | MD-219, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen 90 kDa |
| species reactivity | mouse |
| should not react with | human |
| concentration【浓度】 | ~2 mg/mL |
| technique(s) | flow cytometry: suitable immunocytochemistry: suitable indirect ELISA: suitable microarray: suitable western blot: 0.5-2 μg/mL using whole extract of transfected 293T (human embryonal kidney) cells, expressing mouse MDM2 |
| isotype【同位素/亚型】 | IgG1 |
| UniProt accession no.【UniProt登记号】 | P23804 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | mouse ... Mdm2(17246) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
