Monoclonal Anti-MHC Class II-FITC antibody produced in rat

NA.41

The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Major histocompatibility complex II (MHCII) is specifically localized on antigen presenting cells, such as macrophages, dendritic cells and B cells. MHC class II are classified in to two types classical and non- classical MHC class II molecules. In humans, there are three classical class II molecules HLA-DP, -DQ and –DR and two non-classical molecules, namely HLA-DM and –DO.

Activated C57BL/6 mouse spleen cells

The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 4 μg/mL of sample. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Major histocompatibility complex II (MHCII) plays a vital role in initiating immune response by presenting peptides derived from extracellular pathogens to T cells bearing the CD4 marker. Mutations in the gene increase the risk of susceptibility to autoimmune diseases such as diabetes mellitus, rheumatoid arthritis and pemphigus vulgaris.

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Solution in phosphate buffered saline, pH 7.4, with 15 mM sodium azide.

rat

FITC conjugate

purified immunoglobulin

primary antibodies

M5/114, monoclonal

buffered aqueous solution

mouse

0.5 mg/mL

flow cytometry: suitable

IgG2b

wet ice

2-8℃

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12 - Non Combustible Liquids