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Monoclonal Anti-Myosin (Smooth) antibody produced in mouse

产品编号:3148898
规格:clone hSM-V, ascites fluid
包装规格:100 μL,0.2 ML
产品类别:进口试剂
品牌:Sigma-Aldrich
优惠价:立即咨询
产品价格
产品编号包装单位单价(元)国内现货国外库存询价单
3148898100 μL5140
31488980.2 ML7720
基本信息
MDL number
MFCD00164704
NACRES
NA.41
General description【一般描述】
Monoclonal Anti-Myosin (Smooth) (mouse IgG1 isotype) is derived from the hSM-V hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human uterus smooth muscle extract. Myosin (~500 kDa) is a cytoskeletal protein containing two identical heavy chains (~200 kDa each) and four light chains (15-26 kDa). Myosin molecules consist of two major regions: tail (rod) and heads.
Specificity【特异性】
Mouse monoclonal clone hSM-V anti-Myosin (Smooth) antibody reacts in immunoblotting assays with myosin heavy chain polypeptides of 204 and 200 kDa (SM-1 and SM-2). It does not cross-react with skeletal, cardiac or non-muscle myosin. The antibody stains vascular and visceral smooth muscle cells, as well as cells that have smooth muscle-like characteristics (myofibroblasts and myoepithelial cells). It does not recognize epithelial, endothelial or connective tissue fibroblast cells. The product reacts with smooth muscle tissue from human, dog, rabbit, pig, guinea pig, and chicken.
Immunogen【免疫原】
human uterus smooth muscle extract.
Application【应用】
The antibody may be used in:
  • immunoblotting
  • immunoprecipitation
  • immunohistochemistry
  • immunocytochemistry
  • flow cytometry
  • immunofluorescence

Biochem/physiol Actions【生化/生理作用】
Myosin implicated in cell motility. They aggregate into filaments through the tail region and interact with actin and with ATP through the head region. Myosin molecules spontaneously assemble into filaments in solutions of physiologic ionic strength and pH. In fact, the thick filament consists mainly of myosin molecules. The ATPase of myosin is activated by actin. This activation is the immediate source of the free energy that drives muscle contraction. It binds to the polymerized form of actin, the major constituent of the thin filament. Myosin is generally assumed to be a very specific and reliable smooth muscle marker, provided that the antibody used is specific for smooth muscle myosin and does not cross-react with skeletal, cardiac or non-muscle myosins.
产品性质
Quality Level【质量水平】
200
biological source【生物来源】
mouse
conjugate【偶联物】
unconjugated
antibody form【抗体形式】
ascites fluid
antibody product type
primary antibodies
clone【克隆】
hSM-V, monoclonal
mol wt【分子量】
antigen 200-204 kDa
contains【包含】
15 mM sodium azide
species reactivity
rabbit, pig, human, guinea pig, chicken, canine
technique(s)
immunohistochemistry: 1:500 using and animal frozen section using acetone fixed human.
immunohistochemistry: suitable using using methacarn-fixed, paraffin-embedded sections of human and animal tissue
immunoprecipitation (IP): suitable
western blot: suitable
isotype【同位素/亚型】
IgG1
UniProt accession no.【UniProt登记号】
P35749
shipped in【运输】
dry ice
storage temp.【储存温度】
−20℃
Gene Information
human ... MYH11(4629)
产品说明
Disclaimer【免责声明】
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
安全信息
Storage Class Code【储存分类代码】
12 - Non Combustible Liquids
WGK
WGK 2
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