Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse

MFCD00675266

NA.41

Monoclonal Anti-Nitric Oxide Synthase, endothelial (eNOS) (mouse IgA isotype) is derived from the NOS-E1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized BALB/c mouse. NOS consists of heme and calmodulin binding sites located within its N-terminal half. It has been localized in many different cell types. Based on molecular mass, subcellular location and Ca²⁺ dependence, at least three types of NOS have been classified. Type I NOS (150-160 kD protein) is found in neurons and is also called as neuronal NOS (nNOS), brain NOS (bNOS), cerebral NOS, constitutive NOS or Ca²⁺-regulated NOS (cNOS). Type II (130 kD protein) is found in macrophages and is also known as macrophage NOS (mNOS) or inducible NOS (iNOS). Type III (135 kD protein) is found in endothelial cells and is also called endothelial NOS (eNOS or ecNOS).
The gene encoding endothelial nitric oxide synthase (eNOS) is localized on chromosome 7q35-36 and has 26 exons.

synthetic peptide corresponding to nitric oxide synthase (NOS) from bovine endothelial origin (eNOS, amino acids 1185-1205 with an N-terminally added lysine) conjugated to KLH. The immunogen sequence is highly conserved in human eNOS.

Monoclonal Anti-Nitric Oxide Synthase, Endothelial (eNOS) has been used:

• for enzyme linked immunosorbent assay (ELISA)
• immunoblot
• immunohistochemistry
• immunofluorescence.

Monoclonal Anti-Nitric Oxide Synthase, Endothelial antibody produced in mouse has been used in antibody microarray.

NOS catalyses the oxidation of guanidino nitrogen of arginine. The released nitric oxide mediates vasodilatation, neurotransmission, and antimicrobial and anti-tumor activities. NOS is associated with multiple sclerosis and allergic encephalomyelitis.
Endothelial nitric oxide synthase (eNOS) has been studied as a modulator of vascular function and it is involved in the production of nitric oxide. Defects in the enzyme expression have been shown to be associated with coronary artery disease.

200

mouse

unconjugated

ascites fluid

primary antibodies

NOS-E1, monoclonal

antigen 135 kDa

15 mM sodium azide

mouse, human, bovine, rat

microarray: suitable
western blot: 1:3,000 using whole cell extract of cultured bovine lung endothelial cells

IgA

P29474

dry ice

−20℃

human ... NOS3(4846)
mouse ... Nos3(18127)
rat ... Nos3(24600)

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12 - Non Combustible Liquids

WGK 3