Anti-NONO antibody, Mouse monoclonal

NA.41

Monoclonal Anti-NONO (mouse IgG2a isotype) is derived from the hybridoma NC5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Non-POU-domain-containing, octamer binding protein (NONO), also known as p54nrb, is a member of the DBHS (Drosophila behavior, human splicing) protein family. The family also consists of paraspeckle protein 1 (PSPC1) and splicing factor proline- and glutamine-rich (SFPQ). NONO, PSPC1 and SFPQ are the core protein components of paraspeckles.

Monoclonal Anti-NONO recognizes mouse NONO.

Synthetic peptide corresponding to a sequence at the C-terminal region of mouse NONO, conjugated to KLH.¹ The corresponding sequence is identical in rat and differs by a single amino acids in human.

Monoclonal Anti-NONO antibody produced in mouse has been used in immunohistochemistry and immunoblotting.

Non-POU-domain-containing, octamer binding protein (NONO) complexes with androgen receptor and activates androgen receptor-mediated transcription. NONO is necessary for cAMP-dependent activation of cAMP response element-binding protein (CREB) target genes. It acts as a bridge between the CREB/ transducers of regulated CREB (TORC) complex and RNA polymerase II. NONO associates with Period circadian protein homolog 1 (PERIOD-1) protein and is essential for the maintenance of circadian rhythm. NONO along with the nuclear factor, polypyrimidine tract-binding protein-associated splicing factor (PSF) binds to defective RNA and are capable of their retention.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

200

mouse

unconjugated

purified from hybridoma cell culture

primary antibodies

NC5, monoclonal

buffered aqueous solution

rat, mouse

~1.0 mg/mL

indirect immunofluorescence: 1-2 μg/mL using whole extracts of mouse Hepa1-6 cells.
western blot: 0.5-1.0 μg/mL using whole extracts of mouse Hepa1-6 cells.

IgG2a

Q99K48

dry ice

−20℃

mouse ... Nono(53610)
rat ... Nono(317259)

For continuous use, store at 2-8℃ for up to one month. For extended storage, freeze at -20℃ in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

WGK 2