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单克隆抗 p53 小鼠抗
Anti-p53 antibody, Mouse monoclonal
| 产品编号： | 3149743 |
| 规格： | clone DO-7, purified from hybridoma cell culture |
| 包装规格： | 100 μL，200 μL |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
产品别名
单克隆抗 p53 小鼠抗
Anti-p53 antibody, Mouse monoclonal
Anti-p53 antibody, Mouse monoclonal antibody produced in mouse
Anti-Transformation-related protein 53
Anti-TRP53
抗 TRP53
抗 转化相关蛋白 53
基本信息
| MDL number | MFCD00164709 |
| NACRES | NA.41 |
| General description【一般描述】 | Monoclonal anti-p53, (mouse IgG2b isotype) is derived from the hybridoma DO-7 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on chromosome 17p. The p53 protein is highly conserved and expressed in normal tissues. |
| Application【应用】 | Monoclonal Anti-p53 antibody produced in mouse has been used in:
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| Biochem/physiol Actions【生化/生理作用】 | p53 is a transcription factor that regulates several critical cellular functions such as DNA repair, apoptosis, and cell cycle arrest. p53 primarily functions as a tumor suppressor and inactivates apoptosis inhibitors such as survivin, MCL-1 and BCL-2. Mutations in p53 gene occur most commonly in a wide range of human cancers. Thus, analysis of p53 function has important implications in cancer pathogenesis and therapy . Monoclonal Anti-p53 antibody specifically reacts with p53 in humans. Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21waf1. p21waf1 (cyclin-dependent kinase inhibitor 1A) interacts directly with cyclin dependent kinases, important for cell cycle progression, thereby inhibiting their activity and resulting in cell cycle arrest. |
| Physical form【外形】 | 0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。 |
产品性质
| Quality Level【质量水平】 | 200 |
| biological source【生物来源】 | mouse |
| conjugate【偶联物】 | unconjugated |
| antibody form【抗体形式】 | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone【克隆】 | DO-7, monoclonal |
| form【形式】 | buffered aqueous solution |
| mol wt【分子量】 | antigen ~53 kDa |
| species reactivity | human |
| concentration【浓度】 | ~2 mg/mL |
| technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable indirect ELISA: suitable western blot: 0.1-0.2 μg/mL using human A431 |
| isotype【同位素/亚型】 | IgG2b |
| UniProt accession no.【UniProt登记号】 | P04637 |
| shipped in【运输】 | dry ice |
| storage temp.【储存温度】 | −20℃ |
| Gene Information | human ... TP53(7157) |
产品说明
| Disclaimer【免责声明】 | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
安全信息
| Storage Class Code【储存分类代码】 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
