Anti-phospho-RasGRP1(pThr¹⁸⁴) antibody, Mouse monoclonal

NA.41

Monoclonal Anti-phospho-RasGRP1(pThr184) (mouse IgG2a isotype) is derived from the hybridoma p-RasGRP1-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic phospho-peptide corresponding to residues surrounding Thr184 of human RasGRP1 conjugated to KLH. RAS guanyl releasing protein 1 (RasGRP1) is a member of RasGRP family, characterized by the presence of a N-terminal Ras superfamily guanine nucleotide exchange factor (GEF) domain and and C-terminal calcium- and diacylglycerol (DAG)/phorbol ester-binding domains. It is an intracellular signaling protein. RasGRP1 has a N terminal guanine nucleotide exchange factor domain for Ras-like guanosine triphosphatases (GTPases) (RasGEFN/RasGEF), two EF hand motifs and a carboxy-terminal C1 domain.

a synthetic phospho-peptide corresponding to residues surrounding Thr¹⁸⁴ of human RasGRP1, conjugated to KLH. The corresponding sequence is identical in rat, mouse, monkey, bovine and canine RasGRP1.

Monoclonal Anti-phospho-RasGRP1(pThr184) antibody produced in mouse has been used in immunoblotting and immunoprecipitation.

RAS guanyl releasing protein 1 (RasGRP1) functions as a diacylglycerol (DAG)-regulated nucleotide exchange factor specifically activating Ras through the exchange of bound guanosine diphosphate (GDP) for guanosine triphosphate (GTP). It activates the extracellular signal-regulated kinase (Erk)/mitogen-activated protein (MAP) kinase cascade and regulates T-cells and B-cells development, homeostasis and differentiation.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

200

mouse

unconjugated

purified from hybridoma cell culture

primary antibodies

p-RasGRP1-1, monoclonal

buffered aqueous solution

antigen ~90 kDa

human

~1.0 mg/mL

immunoprecipitation (IP): suitable
western blot: 2-4 μg/mL using whole extracts of HEK-293T cells overexpressing human RasGRP1

IgG2a

O95267

dry ice

−20℃

human ... RASGRP1(10125)
mouse ... Rasgrp1(19419)
rat ... Rasgrp1(29434)

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12 - Non Combustible Liquids