Anti-PIWIL1 antibody, Mouse monoclonal

NA.41

Monoclonal Anti-PIWIL1 (mouse IgG1 isotype) is derived from the hybridoma PIWIL1-6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to keyhole limpet hemocyanin (KLH). The PIWI-like protein 1 (PIWIL1) belongs to the Argonaute protein family. This gene is located on human chromosome 12q24.33. PIWIL1 protein has 861 amino acid residues and possess a molecular weight of 98.6?kDa. It is expressed in prostate, ovary, brain, liver, heart, kidney and skeletal muscle.

synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence is identical in mouse PIWIL1.

Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in various immunochemical techniques including immunoblotting (~95 kDa) and immunohistochemistry.

Piwi proteins and piRNAs have been implicated in epigenetic control of gene expression, transposon silencing and translation regulation. Piwi proteins participates in germline development and gametogenesis of many metazoan species. PIWI-like protein 1 (PIWIL1) is also involved in stem cell proliferation, embryogenesis, growth and development, along with differentiation and maturation in numerous organisms. It can act as a molecular marker for forecasting the prognosis of colorectal cancer (CRC) patients.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

ExtrAvidin is a registered trademark of sigma-aldrich Co. LLC

200

mouse

unconjugated

purified from hybridoma cell culture

primary antibodies

PIWIL1-6, monoclonal

buffered aqueous solution

antigen ~95 kDa

mouse, human

~1.0 mg/mL

immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidin^®-Peroxidase staining system
western blot: 2.5-5.0 μg/mL using whole extracts of mouse testis or HEK-293T cells over-expressing PIWIL1

IgG1

Q96J94

dry ice

−20℃

human ... PIWIL1(9271)
mouse ... Piwil1(57749)

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12 - Non Combustible Liquids