Anti-PIWIL1 antibody, Mouse monoclonal

NA.41

Monoclonal Anti-PIWIL1 (mouse IgG2b isotype) is derived from the hybridoma piwi-like protein 1 (PIWIL1-2) produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to KLH. PIWIL1 is a member of Piwi subfamily. It is expressed mostly to the germline. The encoded protein consists of the PAZ domain and the PIWI domain. The PIWIL1 gene is located on human chromosome 12q24.33.

synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse PIWIL1.

Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in:

• immunofluorescence
• immunoprecipitation
• flow cytometry analysis29}
• immunoblotting
• immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)

Piwi proteins are implicated in epigenetic control of gene expression, transposon silencing and translation regulation. It plays vital roles in the germline development and gametogenesis of many metazoan species.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

ExtrAvidin is a registered trademark of sigma-aldrich Co. LLC

200

mouse

unconjugated

purified from hybridoma cell culture

primary antibodies

PIWIL1-2, monoclonal

buffered aqueous solution

antigen ~95 kDa

human, mouse

~1.0 mg/mL

immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidin^® Peroxidase staining system.
immunoprecipitation (IP): suitable
western blot: 2.5-5.0 μg/mL using whole cell extracts of HEK-293T cells over-expressing PIWIL1.

IgG2b

Q96J94

dry ice

−20℃

human ... PIWIL1(9271)
mouse ... Piwil1(57749)

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