Monoclonal Anti-PSTAIR antibody produced in mouse

MFCD00677382

In every eukaryote examined, CDKs contain an evolutionary conserved 16 amino acid sequence called PSTAIR (EGVPSTAIREISLLKE) which distinguishes them from other protein kinases. The PSTAIR motif is involved in complex formation with cyclins. The availability of monoclonal antibody reacting specifically with the PSTAIR sequence enables the subcellular detection and localization of the various CDKs and examination of substrate interactions, in a variety of organisms.
Monoclonal Anti-PSTAIR (mouse IgG1 isotype) is derived from the PSTAIR hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Cyclin-dependent kinase 1 (CDC2/CDK1) belongs to the Ser/Thr protein kinase family. It is located on human chromosome 10q21.

synthetic 16 amino acid oligopeptide containing the PSTAIR sequence conjugated to BSA.

Mouse monoclonal clone PSTAIR anti-PSTAIR antibody may be used for the localization of cyclin-dependent kinases containing the PSTAIR motif using various immunochemical assays including ELISA, immunoblot and immunoprecipitation.
Monoclonal Anti-PSTAIR antibody produced in mouse has been used in:

• western blot analysis
• enzyme-linked immunosorbent assay (ELISA)
• immunoprecipitation

Cyclin-dependent kinase 1 (CDC2/CDK1) controls various mitotic processes including the reorganization of the cytoskeleton, chromosome segregation, and formation and separation of daughter cells. It is essential for the multiplication of mammalian cell. It participates in tumorigenesis.

200

mouse

unconjugated

ascites fluid

primary antibodies

PSTAIR, monoclonal

antigen 33-34 kDa

15 mM sodium azide

human, lily, Xenopus, eel, Japanese quail, monkey, goldfish, onion, carp, mouse, starfish, amago salmon, ciliates

immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 1:4,000 using a COS-7 cell extract

IgG1

dry ice

−20℃

human ... CDC2(983)
mouse ... Cdc2a(12534)

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12 - Non Combustible Liquids

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