单克隆抗 RPA/p34 小鼠抗

MFCD03845826

NA.41

Monoclonal Anti-RPA/p34 (mouse IgG1 isotype) is derived from the hybridoma 9H8 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified human RPA/p34. Replication protein A Replication protein A (RPA) complex is a single-stranded DNA-binding protein and it is made up of 70-, 32- and 14-kDa subunits.

purified human RPA/p34.

The antibody may be used in:

• immunoblotting (~34 kDa)
• immunocytochemistry
• immunoprecipitation

Replication protein A (RPA) complex is essential for processes like DNA replication, recombination, and DNA repair pathways. The 32-kDa subunit of human RPA is hyper-phosphorylated in its N-terminus upon cellular DNA damage. This phosphorylation causes a change in RPA conformation that down-regulates its activity in DNA replication but does not affect DNA repair processes. Thus the role of RPA phosphorylation in the cellular response to DNA damage is to help regulate DNA metabolism and promote DNA repair.The RPA complex, once bound to single stranded DNA, recruits the ATR (ataxia-telangiectasia mutated and Rad3-related)-ATRIP (ATR-interacting protein) protein kinase complex that is crucial for the cellular response to replication stress and DNA damage and for ATR-mediated Chk1 activation in human cells. Furthermore, the association of this complex to the single stranded DNA causes the phosphorylation of RAD17 protein that is bound to DNA. Yeast strains deficient in RPA are defective in recruiting Ddc2 (yeast homologue of ATRIP) and thus down stream proteins are not phosphorylated. Replication protein A binds with the ssDNA with great affinity and interacts specifically with multiple proteins.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

200

mouse

unconjugated

purified immunoglobulin

primary antibodies

9H8, monoclonal

buffered aqueous solution

antigen ~34 kDa

human, monkey

~2 mg/mL

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.1-0.2 μg/mL using HeLa total cell extract

IgG1

P15927

dry ice

−20℃

human ... RPA2(6118)

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12 - Non Combustible Liquids

WGK 3

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)