Monoclonal Anti-Ryanodine Receptor antibody produced in mouse

MFCD00282461

NA.41

The ryanodine receptor (RyR) is the channel responsible for the release of Ca²⁺ from the sarcoplasmic reticulum (SR) in muscle cells and it also plays a role in Ca²⁺ regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR-1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR-2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).
The antibody reacts strongly with RyR-1 (expressed predominantly in skeletal muscle and areas of the brain; also known as the α isoform) and RyR-2 (expressed predominantly in the heart muscle, but also found in stomach, endothelial cells and diffuse areas of the brain; also known as the β isoform). In immunohistochemistry, the antibody stains are consistent with the endoplasmic reticulum localization of the receptor in rat hippocampus.

partially purified chicken pectoral muscle ryanodine receptors (RyR)

Mouse monoclonal clone 34C anti-Ryanodine receptor is used to tag ryanodine receptors for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques. It is used as a probe to determine the presence and roles of ryanodine receptor in studies of calcium flux from the sarcoplasmic reticulum of muscle cells.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Purified IgG1 antibody containing 0.05% sodium azide.

200

mouse

unconjugated

ascites fluid

primary antibodies

34C, monoclonal

antigen 565 kDa (non-mammalian vertebrates, doublet at 565 kDa representing the α and β isoforms)

rabbit, fish, canine, sheep, frog, bovine, primate, mink, chicken, rat, human, mouse

direct immunofluorescence: suitable
immunohistochemistry (frozen sections): 1:1,000
immunoprecipitation (IP): suitable
western blot: 1:5,000

IgG1

P21817

dry ice

−20℃

human ... RYR1(6261), RYR2(6262)
mouse ... Ryr1(20190), Ryr2(20191)
rat ... Ryr1(114207), Ryr2(84025)

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Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)