Monoclonal Anti-S100A6 antibody produced in mouse

MFCD00677384

NA.41

Monoclonal Anti-S100A6 (Calcyclin) (mouse IgG1 isotype) is derived from the CACY-100 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. S100 calcium-binding protein A6 is mapped to human chromosome 1q21.3. S100A6 has been shown to possess 43-47% homology with S-100α, S-100β, and S100A2 (S100L).

Recognizes an epitope located on S100A6 (Calcyclin in the old terminology) in a Ca²⁺ ion-dependent manner. The product does not react with other members of the EF-hand family such as calmodulin, parvalbumin, intestinal calcium-binding protein, S100A2 (S100L), caltropin, the α chain of S-100 (i.e. in S-100a and S-100ao), or the β chain (i.e. in S-100a and S-100b).

Ca²⁺-binding proteins from pig stomach tissue

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

S-100 proteins are calcium-modulated proteins that bind calcium and zinc ions reversibly at physiologic pH and ionic strength, followed by a conformational change in the molecule. S100A6 along with other S100 family proteins bindsprotein phosphatase 5(PP5) in a calcium-dependent manner.

200

mouse

unconjugated

ascites fluid

primary antibodies

CACY-100, monoclonal

15 mM sodium azide

goat, human, bovine, canine, rat, lizard, pig

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable using native preparations
indirect immunofluorescence: 1:4,000 using bovine tongue frozen sections
western blot: suitable using denatured-reduced preparations

IgG1

P06703

dry ice

−20℃

human ... S100A6(6277)
rat ... S100a6(85247)

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

12 - Non Combustible Liquids

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