Monoclonal Anti-TSG101 antibody produced in mouse

NA.41

Tumor susceptibility gene 101 (TSG101) is an endosomal sorting complex required for transport (ESCRT) component, present on endosomal membranes. This gene is located on chromosome 11p15. The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a leucine-zipper motif within a coiled-coil domain near the carboxyl terminus.

Amino acids 167-374 of TSG101 protein expressed in E. coli.

Suggested starting dilutions are as follows: WB: 1:500-1:3000. Suggested fixation for ICC/IF: 3-3.5% PFA with 0.05% saponin treatment. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Monoclonal Anti-TSG101 antibody has been used in western blotting and immunoprecipitation (IP) analyses.

Tumor susceptibility gene 101 (TSG101) is essential for the generation of central supramolecular activation cluster, and the signal termination of T cell receptor (TCR) microclusters (MC). It plays an important role in TCR downregulation and separation of TCR- major histocompatibility complex (MHC)-peptide from signaling complexes, that are enriched with protein kinase C θ (PKCθ).
TSG101-4A10 recognizes TSG101 protein, the product of a recently identified tumor susceptibility gene whose inactivation in mouse fibroblasts results in cell transformation and the ability of those cells to form tumors in nude mice.
The TSG101 gene encodes a 46 kDa protein and contains a proline-rich domain and a luecine-zipper motif within a coiled-coil domain near the carboxyl terminus.

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Phosphate-buffered saline, no preservative added.

100

mouse

unconjugated

purified immunoglobulin

primary antibodies

4A10
monoclonal

liquid

46 kDa

mouse, monkey, hamster, human, rat

1 mg/mL

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1:500-1:3000

IgG1

Q99816

wet ice

−20℃

human ... TSG101(7251)

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