抗-VSV糖蛋白单克隆抗体

NA.46

Monoclonal Anti-VSV Glycoprotein (mouse IgG1 isotype) is derived from the P5D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide of vesicular stomatitis virus glycoprotein (VSV-G), conjugated to keyhole limpet hemocyanin (KLH). VSV-G is a protein expressed on the surface of bullet shaped virions.

该抗体可识别包含VSV糖蛋白的五个羧基末端氨基酸的表位。在受感染的细胞中，该抗体将未成熟型VSV-G定位在粗糙内质网(RER)和高尔基复合体潴泡中，将成熟型VSV-G定位在细胞表面和出芽病毒中。该抗体不会染色缺少膜和细胞质结构域的分泌型VSV-G。该抗体可用于微注射抗体、免疫印迹、免疫沉淀、免疫细胞化学和免疫电镜法研究新合成的VSV-G在向质膜和细胞表面转移过程中细胞质结构域的作用。该抗体可用于对以VSV-G羧基末端标记的外源性导入结构进行检测、免疫沉淀和免疫细胞化学染色。该标记不会干扰所研究蛋白质的功能，可被P5D4抗体特异性识别，而不与任何内源性蛋白质发生交叉反应。

KLH标记的含有水泡性口炎病毒糖蛋白(VSV-G)的15个羧基末端氨基酸(497-511)的合成肽。

A dilution of 1:1000 of Monoclonal Anti-VSV Glycoprotein-Cy3^® antibody produced in mouse was used to detect VSV glycoprotein in pig sperms by immunofluorescence.
Monoclonal Anti-VSV Glycoprotein Cy3^™ antibody produced in mouse has been used:

• in studies applying
• microinjection of antibody
• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunoelectron microscopy.

The envelope of vesicular stomatitis virus (VSV) consists of a bilayer membrane with a single type of glycoprotein, the G-protein (VSV-G) which mediates attachment to the cell surface and induces pH-dependent fusion between viral and target membranes. The carboxyl terminus of the VSV-G protein which does not have any homology with cellular proteins, has been engineered into expression vectors as a tag. Proteins expressed with this tag may thus be detected and localized using an antibody reactive specifically against this epitope with no risk of cellular background staining.
The vesicular stomatitis virus (VSV) glycoprotein facilitates the entry of HIV into the CD4 T cells through endocytotic pathway. The coupling of Cy3 to Anti-VSV Glycoprotein antibody allows for the visualization of protein by fluorescent microscopy.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Cy is distributed under license from Amersham Biosciences Limited.
Cy is a registered trademark of Cytiva
Cy3 is a trademark of Cytiva

200

mouse

CY3 conjugate

purified immunoglobulin

primary antibodies

P5D4, monoclonal

buffered aqueous solution

direct immunofluorescence: 1:10,000 using COS-7 cells transfected with a VSV-G tagged vinculin construct

IgG1

wet ice

2-8℃

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