Anti-WASH1 antibody, Mouse monoclonal

NA.41

Monoclonal Anti-WASH1 (mouse IgG1 isotype) is derived from the hybridoma WASH1-27 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human WASH1. WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog), a new member of the Wiskott−Aldrich Syndrome protein (WASP) family. Similar to other WASP family members, it contains a C-terminal for ′WH2, connecting and acidic′ (WCA) domain that binds to actin and the Arp2/3 complex. In addition, WASH1 also contains a short proline-rich region, a unique N-terminal domain termed WASH-homology domain (WAHD1), and a tubulin-binding region. Through its WAHD1 region, it interacts with family with sequence similarity 21 (FAM21), a protein that links WASH1 to endosomes. WASH1 forms part of a multiprotein complex composed of FAM21, KIAA1033 strumpellin and WASH-interacting protein (SWIP) and coiled coil domain containing 53 (CCDC53).

a synthetic peptide corresponding to an internal region of human WASH1 (GeneID: 100287171), conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.

Monoclonal Anti-WASH1 antibody produced in mouse has been used in may be used in various immunochemical techniques including immunoblotting (~72 kDa), immunoprecipitation and immunofluorescence.

WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog), is a nucleation-promoting factor at the surface of endosomes. It recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of endosomes. It associates with tubulin and localizes to early and recycling endosomes, where together with the Arp2/3 complex and actin, it is required for maintaining the shape of the endosomal compartment and the regulation of the retrograde transport.

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

200

mouse

unconjugated

purified from hybridoma cell culture

primary antibodies

WASH1-27, monoclonal

buffered aqueous solution

antigen ~72 kDa

human, mouse

~1 mg/mL

immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells
western blot: 2.5-5.0 μg/mL using whole extracts of HEK-293T cells overexpressing mouse WASH1

IgG1

dry ice

−20℃

human ... WASH1(100287171)

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12 - Non Combustible Liquids