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产品性质
biological source【生物来源】 | human ovary |
growth mode【生长模式】 | Adherent |
karyotype【核型】 | 2n = 46, pseudodiploid |
morphology【形态学】 | Epithelial |
products【产品】 | Not specified |
receptors【受体】 | Not specified |
technique(s) | cell culture | mammalian: suitable |
relevant disease(s)【相关疾病】 | cancer |
shipped in【运输】 | dry ice |
storage temp.【储存温度】 | −196℃ |
基本信息
Cell Line Origin【细胞系来源】 | Human Caucasian ovary teratocarcinoma |
Cell Line Description【细胞系描述】 | Derived from ascitic fluid cells of a 12 year old Caucasian female with teratocarcinoma of the ovary. The cells produce tumours in nude mice. |
Application【应用】 | Tumourigenicity studies |
DNA Profile【DNA图谱分析】 | STR-PCR Data: Amelogenin: X CSF1PO: 9,12 D13S317: 9,10 D16S539: 9,12 D5S818: 11 D7S820: 9 THO1: 7,9 TPOX: 11 vWA: 15,17 |
Culture Medium【培养基】 | EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Heat Inactivated). |
Subculture Routine【传代培养常规】 | Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37℃. |
Other Notes【其他说明】 | Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information. |