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Papain Agarose from papaya latex
| 产品编号： | 3179106 |
| 规格： | lyophilized powder, 90-150 units/mL packed gel |
| 包装规格： | 25 UNITS，50 UNITS，100 UNITS |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| MDL number | MFCD00131791 |
| NACRES | NA.54 |
| Application【应用】 | Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P4406 is agarose-linked papain and is provided as a lyophilized powder stabilized with lactose. Product P4406 has been used to digest purified anti-JMJD6 hamster monoclonal antibody into Fab fragments. Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. |
| Biochem/physiol Actions【生化/生理作用】 | Papain papaya latex has antifungal activity against C. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine. . The pH optimum is 6.0-7.0 . Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme. A cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. pH optimum 6.0-7.0 Also hydrolyzes esters and amides. |
| Unit Definition【单位定义】 | One unit will hydrolyze 1.0 μmole of BAEE per min at pH 6.2 at 25℃, unless otherwise indicated below. |
| Physical form【外形】 | Lyophilized powder, stabilized with lactose |
产品性质
| form【形式】 | lyophilized powder |
| Quality Level【质量水平】 | 200 |
| Specific Activity | 90-150 units/mL packed gel |
| mol wt【分子量】 | 21 kDa |
| extent of labeling【标记范围】 | 3,000-5,000 units per g agarose (at pH7.0 at 30 ℃.) |
| matrix【基质】 | beaded agarose |
| storage temp.【储存温度】 | −20℃ |
| packaging【包装】 | 25, 50, 100 units in glass bottle |
安全信息
| Pictograms【象形图】 | GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H317 - H334 |
| Precautionary Statements | P261 - P280 - P342 + P311 |
| Hazard Classifications【危险分类】 | Resp. Sens. 1 - Skin Sens. 1 |
| Storage Class Code【储存分类代码】 | 13 - Non Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | dust mask type N95 (US), Eyeshields, Faceshields, Gloves |
