含有Taq DNA聚合酶的PCR核心试剂盒

32161000

NA.55

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. This enzyme is in a recombinant form, expressed in E. coli. Taq DNA polymerase enzyme can withstand high temperatures up to 95 ℃ without significant loss of activity. Taq DNA Polymerase is a magnesium ion-dependent enzyme. Taq DNA Polymerase possesses 5′→3′ DNA polymerase activity and 5′→3′ exonuclease activity.

CORET PCR kit has been used for the amplification of genomic DNA during the study of genetic diversity in various species of Manilkara zapota.
PCR Core kit with Taq DNA polymerase is suitable for the amplification of genomic DNA, cDNA, or cloned DNA templates. Each component has been tested in the polymerase chain reaction. Optimal concentrations of Taq polymerase, template DNA, primers, and MgCl₂ will depend on the specifics of the system. It may be necessary to optimize each component separately, especially the Taq and Mg²⁺ concentrations and the cycling parameters.

试剂盒中含有足够用于250单位Taq DNA聚合酶的试剂。

以下试剂必需但并未提供在试剂盒内：

• 矿物油，M 8662(可选)
• 热循环仪
• 引物
• 待扩增的DNA
• 氯仿，C 7559(可选)

No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

组份可单独销售
P2192 10X PCR Buffer, Optimized for routine PCR with MgCl₂ included 1 mL/vial
P2317 10X PCR Buffer without MgCl₂, Optimized for routine PCR without MgCl₂ 1 mL/vial
M8787 Magnesium chloride solution, PCR Reagent, 25 mM MgCI₂ solution for PCR 1 mL/vial
W1754 Water, PCR Reagent 3 vial(s)
D7295 Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .25 mL

200

liquid

sufficient for 250 reactions

dNTPs included
hotstart: no

PCR: suitable

colorless

purified DNA

wet ice

−20℃

10 - Combustible liquids

WGK 3