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Peroxidase, Maleimide Activated from horseradish
| 产品编号： | 3180408 |
| 规格： | lyophilized powder, >200 units/mg protein |
| 包装规格： | 2 MG，3 X 2 MG |
| 产品类别： | 进口试剂 |
| 品牌： | Sigma-Aldrich |
| 优惠价： | 立即咨询 |
基本信息
| MDL number | MFCD00284131 |
| NACRES | NA.54 |
| General description【一般描述】 | Peroxidase (POD) is an enzyme that includes a group of specific enzymes such as nicotinamide adenine dinucleotide (NAD) peroxidase, NADP peroxidase, fatty acid peroxidase and a group of non-specific enzymes from various sources. It is present in animals, higher plants and other organisms. |
| Application【应用】 | For direct conjugation to compounds containing free sulfhydryl groups for use as an enzyme-linked detection reagent. Instructions for use included. In a study on horseradish peroxidase, the Maleimide compounds succinimidyl 4-maleimidobutyrate and succinimidyl 6-maleimidohexanoate have been shown to be the most effective in the conjugation of Fab′ to horseradish peroxidase. This enzyme has also been used in a study to investigate the effectiveness in staining a mitochondrial protein, lipoamide dehydrogenase. To increase signal intensity of immunoconjugates, immunoglobulins were synthetically modified to contain multiple primary amines and covalently coupled to maleimide activated peroxidase. This can result in more efficient conjugation and signal amplification. |
| Biochem/physiol Actions【生化/生理作用】 | Peroxidase (POD) helps in the dehydrogenation of several organic compounds like phenols, aromatic amines, hydroquinones etc. |
| Other Notes【其他说明】 | Type VI peroxidase activated with maleimidocaproic acid N-hydroxysuccinimide ester. |
| Unit Definition【单位定义】 | One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 seconds at pH 6.0 at 20℃. |
| Physical form【外形】 | Lyophilized powder containing trehalose and sodium citrate |
产品性质
| form【形式】 | lyophilized powder |
| Quality Level【质量水平】 | 200 |
| Specific Activity | >200 units/mg protein |
| composition【组成】 | Protein, ~50% E |
| extent of labeling【标记范围】 | 1-3 mol maleimide per mol protein |
| storage temp.【储存温度】 | −20℃ |
安全信息
| Pictograms【象形图】 | GHS08 |
| Signal word【警示用语：】 | Danger |
| Hazard Statements | H317 - H334 |
| Precautionary Statements | P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313 |
| Hazard Classifications【危险分类】 | Resp. Sens. 1 - Skin Sens. 1 |
| Storage Class Code【储存分类代码】 | 11 - Combustible Solids |
| WGK | WGK 3 |
| Personal Protective Equipment【个人防护装备】 | dust mask type N95 (US), Eyeshields, Gloves |
