Polynucleotide Kinase from T4-infected Escherichia coli

2.7.1.78 ( BRENDA | IUBMB )

MFCD00166009

NA.53

Suitable for:

• Sequencing or nucleic acid tagging (DNA and RNA) by 5′-end labeling
• 5′ phosphorylation of oligonucleotides
• Removal of 3′-phosphate groups from phosphorylpolynucleotides

T4 Polynucleotide Kinase is supplied in a solution of 50% glycerol (v/v), 20 mM Tris-HCl (pH 7.5), 25 mM KCl, 2mM DTT, 0.1 mM EDTA, and 0.1 μM ATP.

Polynucleotide kinase catalyses a "forward reaction" transfer of the γ-phosphate of ATP to the 5′ hydroxyl terminus of single- and double-stranded nucleic acids (DNA and RNA) and 3′-nucleoside monophosphates. In exchange reactions containing ADP, the enzyme will catalyze the exchange of 5′-terminal phosphate groups and ATP. The 3′-phosphatase activity enables the enzyme to remove 3′-phosphoryl groups from phosphorylpolynucleotides.
1. Forward reaction: Transfer of the labeled γ-phosphate from [γ-³²P]-ATP to the free 5′-hydroxyl group of the substrate.
5′-HO-DNA + [γ-³²P]-ATP → 5′-³²PO-DNA + ADP.
Substrates that do not have a free 5′-hydroxyl require prior dephosphorylation by alkaline phosphatase.
2. Exchange reaction: First, the terminal 5′-phosphate is transferred from the substrate to ADP present in the reaction mixture. Then, the labeled γ-phosphate from [γ-³²P]-ATP is transferred to the free hydroxyl group of the substrate.
5′-PO-DNA + ADP → 5′-HO-DNA + ATP
5′-HO-DNA + [γ-³²P]-ATP → 5′-³²PO-DNA + ADP

One unit catalyzes the transfer of one nanomole of ³²P to the 5′-end of micrococcal nuclease-treated DNA in 30 min. at 37 ℃. Transfer is detected as incorporation into acid-insoluble material.

Activity is determined in a reaction mixture containing 40 mM Tris-HCl (pH 7.5), with 10 mM MgCl2, 5 mM dithiothreitol, 0.5 mM 5′-OH polynucleotide ends, and mM [γ-32P]-ATP.

200

for molecular biology

buffered aqueous glycerol solution

33 kDa

10 units/μL

Endonuclease and exonuclease, none detected

wet ice

−20℃

GHS08

Danger

H334

P261 - P284 - P501

Resp. Sens. 1

10 - Combustible liquids

WGK 3

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)