PRMT1 from rat

NA.32

Methyl transferases catalyze the addition of methyl groups to nitrogen, carbon, sulfur, and oxygen atoms of small molecules, lipids, proteins, and nucleic acids. Eight mammalian protein arginine methyltransferases (PRMT) have been identified. PRMT1 is the predominant member of the methyl transferases, which catalyzes the protein arginine N-methylation reactions. PRMT1 is implicated in various cellular processes including: transcription, RNA processing, and signal transduction.

The specific activity is ≥ 0.1 nmol/mgP/min. measured by ³H-AdoMet incorporation into histone for 30 minues at 30 ℃.

Solution of 50 mM Tris, pH 7.6, 5 mM DTT, 0.2% IGEPAL^® CA-630, 150 mM NaCl, and 30% glycerol (w/v).

The N-methyltransferase activity is determined by detecting the level of radiolabel transfer from ³H-AdoMet (Methyl donor) to histone (Cat. No. H4380), which is arginine rich (methyl acceptor).

IGEPAL is a registered trademark of Solvay

200

expressed in E. coli

≥90% (SDS-PAGE)

buffered aqueous solution

Q63009

dry ice

−20℃

rat ... Prmt1(60421)

10 - Combustible liquids

WGK 1

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)